【文獻標(biāo)題】MiRNA-34c-5p protects against cerebral ischemia/reperfusion injury:involvement of anti-apoptotic and anti-inflammatory activities

【作者】Yaoran Tu1 & Yong Hu

【作者單位】南昌大學(xué)（Nanchang University）

【文獻中引用產(chǎn)品】

β-actin抗體

抗兔二抗—抗體

【關(guān)鍵詞】miR-34c-5p . Cerebral ischemia . Inflammation . Apoptosis

【DOI】10.1007/s11011-021-00724-5

【影響因子(IF)】3.58

【出版期刊】《Metabolic Brain Disease》

【產(chǎn)品原文引用】

Western blotting was performed as previously described (Sayre et al. 2010). Protein concentrations were quantified using the BCA Protein Assay Kit. After denaturing in boiling water for 10 min, protein samples were separated using SDSPAGE gel (10%), followed by transferring to PVDF membrane. The membrane was incubated with anti-P65 antibody (1:500, Youliante, Wuhan, China), Bcl-2 antibody (1:1000, Youliante, Wuhan, China), Bax antibody (1:1000, Youliante, Wuhan, China), COX-2 antibody (1:500, Youliante, Wuhan, China), iNOS antibody (1:500, Youliante, Wuhan, China), NCOA1 antibody (1:500, Youliante, Wuhan, China) and β-actin antibody (1:1000, Hengyuan, Shanghai, China) overnight. Then anti-rabbit secondary antibody (1:1000, Hengyuan, Shanghai, China) was added for incubation of another 1 h.

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