【文獻標題】Enantioselective toxicity and oxidative stress effects of acetochlor on earthworms (Eisenia fetida) by mediating the signaling pathway

【作者】Yalei Liu, Kuan Fang, Xiaolian Zhang,et.al

【作者單位】中國農(nóng)業(yè)科學院煙草研究所（Tobacco Research Institute of Chinese Academy of Agricultural Sciences）

【文獻中引用產(chǎn)品】

蚯蚓8-羥基脫氧鳥苷（8-OHdG）ELISA試劑盒

蚯蚓細胞色素P450(CYP450)ELISA試劑盒

蚯蚓羥基自由基(OH)ELISA檢測試劑盒

【關鍵詞】Acetochlor,Enantiomer,Earthworm,Toxicity,Transcriptomic

【DOI】doi.org/10.1016/j.scitotenv.2020.142630

【影響因子(IF)】8.6

【出版期刊】《Science of the Total Environment》

【產(chǎn)品原文引用】

The OH content, 8-OhdG content, and CYP450 content were determined according to the method of Zhang et al. (2020) using a kit obtained from the ShangHai HengYuan Biological Technology Co., Ltd. (Shanghai, China). The OH content, 8-OHdG content, and activity of P450 in earthworms were detected by the double antibody sandwich method. The OH in earthworms combined with the antibodies of OH marked by HRP (Horseradish Peroxidase). Then, 3,3′,5,5′-tetramethylbenzidine (TMB) was added to produce a chromogenic reaction with the compound of antibody-antigen-antibody labeled by the enzyme. The absorbance of the reaction solution was recorded at

450 nm. The detection principle and method of 8-OhdG and CYP450 are the same as those of OH.

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